NMR Spectrometer: Applications, Components and Functions

NMR Spectrometer: Applications, Components and Functions Describe briefly the major components of a NMR spectrometer and their function. The Magnet The capability of an NMR instrument is critically dependent upon the magnitude and homogeneity of the static magnetic field and on the bore size of the magnet. There are three main types of magnet; permanent, resistive, and superconducting. (Gadian, 2004) The Gradient System The generation of magnetic resonance images relies on the appropriate use of pulsed magnetic field gradients. These gradients are generated in the same way as those produced by the shim coils, i.e. by specially constructed coils mounted within the bore of the magnet, designed to produce field gradients of the required strength and linearity. (Gadian, 2004) The Transmitter The transmitter generates radiofrequency pulses of the appropriate frequency, power, shape, and timing. It contains a frequency generator, a waveform generator shape the pulses as required, a gate which switches the transmission on and off at the required times, and a power amplifier which boosts the radiofrequency power to the values that are required in Fourier-transform NMR. (Gadian, 2004) The Radiofrequency coil(s) The Radiofrequency coils are used for transmitting the B1 field into the region of interest, and for detecting the resulting signal. In some cases, the same coil is used for transmission and reception, while in others it may be preferable to use separate transmit and receive coils. (Gadian, 2004) The Receiver The design of a modern digital receiver centres around an analog to digital converter (ADC), which samples the analog NMR signal and converts it into digital format. Important characteristics of the ADC are its conversion bandwidth and resolution. The Computer The computer has a wide range of function. Its main functions are: (i) to control the radiofrequency and field gradient pulses; (ii) to accumulate the data; and (iii) to process and display the data. (Gadian, 2004) The magnet produces the Bo field necessary for the NMR experiments. When nuclei interact with a uniform external magnetic field, they behave like tiny compass needles and align themselves in a direction either parallel or anti parallel to the field. The two orientations have different energies, with the parallel direction having a lower energy than the anti parallel. Immediately within the bore of the magnet are the shim coils for homogenizing the Bo field. Within the shim coils is the probe. The probe contains the Radiofrequency (RF) coils for producing the B1 magnetic field necessary to rotate the spins by 90o or 180o. This will be done by the RF transmitter shown in figure 1. The RF coil also detects the signal from the spins within the sample. These signals will be detected by the RF receiver in figure1. The sample is positioned within the RF coil of the probe. Some probes also contain a set of gradient coils. These coils produce a gradient in Bo along the X, Y, or Z axis. The heart of the spectrometer is the computer. It controls all of the components of the spectrometer. The RF components under control of the computer are the RF frequency source and pulse programmer. The source produces a sine wave of the desired frequency. The pulse programmer sets the width, and in some cases the shape, of the RF pulses. The RF amplifier increases the pulses power from milli Watts to tens or hundreds of Watts. The computer also controls the gradient pulse programmer which sets the shape and amplitude of gradient fields. The gradient amplifier increases the power of the gradient pulses to a level sufficient to drive the gradient coils. The operator of the spectrometer gives input to the computer through a console terminal with a mouse and keyboard. Some spectrometers also have a separate small interface for carrying out some of the more routine procedures on the spectrometer. A pulse sequence is selected and customized from the console terminal. The operator can see spectra on a video display located on the console and can make hard copies of spectra using a printer. Comment on the nature, volume, condition, etc. required of a sample for nmr studies on biofluids. An important aspect of conducting NMR spectroscopy on biological fluids and tissues is suppression of large interfering resonances, in particular from water, buffers and cosolvents (in the case of extracts). It is also important to be able to apply accurately shaped (non-rectangular) r.f pulses and/or magnetic field gradients across samples to enable diffusion measurements, multidimensional NMR experiments, and the latest solvent suppression approaches. (Gadian, 2004) In any kind of NMR probe, there are two sample volumes to consider. First is the total volume of sample required (the sample volume) and second is the active volume or the volume of sample that is exposed to the r.f coils. For probes with the commonly used saddle coil, the ratio of active/sample volume is ~0.5. Typical sample volumes for metabonomics applications range from 120 to 500  µl, a range that is normally adequate for commonly available biofluids such as urine or plasma from anything larger than a mouse. There are also numerous examples of small volume probes (1-30 µl) that could have potential uses in certain applications on rare or hard to-obtain biofluids such as CSF or synovial fluids from small laboratory animals. (Gadian, 2004) No pre-treatment of the sample is required. The metaobiltes which are present in sufficiently mobile form and at sufficient concentration to give detectable signals. For in vivo studies a minimum concentration of 0.2mM is normally required. The amount of sample to be analysed itself is limited by instrument/magnet design but for simple solution studies a typical maximum volume is 0.5 -1 ml. For the less sensitive elements therefore it is desirable to have more concentrated solutions Hydrogen NMR spectra can be obtained in less than one minute depending on concentration of analytes in sample. 8 combined scans (each of 1-2 seconds duration) is usually enough to give a clear signal. Other nuclei are less sensitive and require more combined scans eg 13C can require a few hours of repeated scanning before signals are clear. Comment on technical aspects such solvent interferences, exchangeable Hydrogens,experiment duration, etc that are specific/relevant to NMR of biofluids. The presence of a water (HDO) peak will only serve to degrade the quality of NMR spectra. The concentration of water in an aqueous solution is about 55M and therefore the signal from water itself usually dwarfs/masks weaker signals. in a normal spectrum but a technique of water-suppression is commonly used to reduce the dominance of this peak and protons in the sample that exchange with water. In order to remove any interferences from solvent signals during NMR analysis, solvent suppression techniques are employed, the main ones being presaturation and WET (Water suppression Enhanced through T1 effects). The former is a long-standing method that uses shaped pulses to saturate the solvent resonance(s). The WET method uses selective pulses to excite the solvent resonances then dephasing gradient pulses to destroy them. The two techniques take 0.5-2 s and 50-100 ms, respectively, so the WET method is preferred for continuous-flow NMR. The time to acquire a spectrum depends most critically the number of accumulated scans and hence on the sensitivity of the nucleus under investigation and correspondingly the concentration of the sample. In general, as molecules become increasingly immobilized they produce broader signals. Therefore spectra of living systems revel narrow signals from metabolites which have a high degree of molecular mobility, whereas macromolecules, which are highly immobilized (such as DNA and membrane phospholipids), produce very much broader signals.1 H NMR spectroscopy imposes particularly stringent requirements. High field spectrometers that are used for studies of solutions may have field homogeneity as 1 part in 109, although of course this is over a much smaller sample volume (e.g. 0.5ml) than the volumes characteristic of in vivo studies. Much better spectral resolution can be achieved using high field system study relatively small volumes of body fluids or of cell or tissue extracts. A great deal of information can be derived from such studies. (Gadian, 2004) The poor sensitivity of NMR imposes limitations on the concentrations of compounds that can be detected, and upon the spatial resolution that can be achieved. Because of the large number of variables, it is difficult to give anything other than an order-of-magnitude estimate for the concentrations that are required and for the spatial resolution that can be achieved. Typically, however, we can anticipate that, for metabolic studies in vivo, minimum concentrations of 0.2mM and above will be required in order for a metabolite to give a detectable signal. One of the most remarkable features of magnetic resonance is the extensive range of pulse sequences that have been developed, with a view to enhancing the quality and information content of spectra. For example, innovative pulse sequences have contributed in many ways to improvements in image contrast, spectral localization, suppression of unwanted signals, and visualization of specific structural, biochemical, or functional properties. The existence of the chemical shift enables us to use NMR to distinguish not only between different molecules, but also between individual atoms within a molecule. When used in conjunction with intensity measurements and spin-spin coupling data, chemical shifts of the spectral lines of a molecules provide a great deal of information about its structure. (Gadian, 2004) Identify the major observable components in the control samples of human urine (see 1H spectrum obtained for a healthy adult at the session and compare with that of the 7 month old child in the Canavans disease case study in the lecture notes) Creatinine (Crn) is already identified for you. Canavans disease is an autosomal recessive disorder in which spongy degeneration of white matter is observed. Several groups have shown a large increase in the NAA/Cr and NAA/Cho ratios in children with Canavans disease, consistent with enzyme deficiency. The metabolites monitored were those that are present in sufficiently mobile form and at sufficient concentration to give detectable signals. The urine of patients with Canvans disease shows an unusual signal that can be attributed to NAA. Quantification of this signal from timed urine samples allows an assessment of the rate at which NAA is being removed from the brain. Sketch the molecular structures of each of the major components in urine and of Vitamin C. For each molecule indicate which hydrogen atoms are likely to give rise to distinct signals in a water suppressed 1H NMR spectrum (repeat for Vit C and compare with its reference spectrum provided) Indicates which hydrogen atoms are likely to give rise to distinct signals in a water suppressed 1H NMR spectrum Components in urine There are four different types of H but only two signals as two are bound to N Creatinine (Crn) Betaine (Bet) Hippuric acid (Hip) Acetate (Ace) Lactic acid (Lac) Alanine (Ala) Citrate (Cit) Oxalic acid (Ox) Ascorbic Acid (Vitamin C) (not normally present in urine!) There are six different types of H but only two signals as four are bound to O Identify the major spectral changes observed in the spectrum of urine obtained after ingestion of 10g/day Vitamin C over three days. What information do these spectra provide on the extent of Vitamin C metabolism and on the identities of the major excreted metabolites this is important do not gloss over it. The crn peak stays consistent throughout the 3 day period, as do the other excreted metabolites (Hip, Bet, Cit, Ace). This suggests Vitamin C has no effect on the excretion of other metabolites. The standard and healthy dosage of Vit c is 75 milligrams per day. Therefore at this dosage there is excess Vit c which is unmetabolised and excreted in the urine as shown in fig 4. The diagrams in figure 4 show more Vit c being excreted with each day that passes. Using the standard it is clear to see there is an increase in the peak at the position associated with vitamin C. The area around the peak also generates several smaller peaks. These are not vitamin C but are products with similar structures. These will probably be intermediates in the pathway which breaks down ascorbate acid and contain the same CH2-CH molecular unit intact that was present in the parent ascorbate structure, and this is the bit that gives the NMR fingerprint. Ascertain (Web of Knowledge or similar search would be appropraite) the generally agreed metabolites (excreted or otherwise) of Vitamin C (there are more than two and this is probably the most important aspect of the report so it needs some investigation!) Discuss whether these could and/or would be identified in the 1H nmr spectrum of urine after a prolonged high dosage of vitamin C. What common feature persists throught the degradative pathway- does this match your results? The generally agreed metabolites of Vit C are dehydroascorbate (DHAA), 2-O-methyl ascorbate, 2-ketoascorbitol as well as those in figure 5 (L-Threonic acid, Oxalic acid, Lactic acid). ASCORBATE BREAKDOWN Dehydroascorbate, if not reduced back to ascorbate, decomposes with a half-life of a few minutes, since this  compound is unstable at physiologic pH. The product of the hydrolysis is 2,3-diketo-L-gulonate, which does not possess antiscorbutic effects any more. 2,3-diketo-L-gulonate is decarboxylated to L-xylonate and L-lyxonate. These 5-carbon compounds can enter the pentose phosphate pathway and the L- to D-conversion is suggested to occur through xylitol. Another minor pathway of ascorbate catabolism is a carbon chain cleavage yielding oxalate and 4-carbon intermediates. Pentose phosphate pathway enters the glycolytic/gluconeogenic sequence at triose phosphates and fructose-6-phosphate. Ascorbate and dehydroascorbate, according to the previous assumptions, can be rapidly metabolized to glucose in isolated murine hepatocytes and in HepG2 cells. When glutathione-dependent recycling is inhibited by the oxidant menadione or by the glutathione synthesis inhibitor buthionine sulfoximin e, gluconeogenesis from ascorbate is stimulated. The participation of the non-oxidative branch of the pentose phosphate pathway has been demonstrated by the administration of oxythiamine, a thiamine antagonist which inhibits transketolases. In hepatocytes gained from oxythiamine-treated mice glucose production from dehydroascorbate is lower, and a pentose phosphate cycle intermediate, xylulose-5-phosphate is accumulated. This path of ascorbate catabolism could be demonstrated even in cells unable to synthesize ascorbate, i.e., in cells of human origin and in non-hepatic murine cells. In murine and human erythrocytes-which are unable to synthesize glucose (glucose-6-phosphatase is lacking)-ascorbate or dehydroascorbate addition resulted in the increase of lactate, the end product of anaerobic glycolysis. Lactate production could be stimulated by the addition of menadione or inhibited by oxythiamine treatment of the cells indicating that the pentose phosphate pathway is involved in as corbate catabolism both in hepatocytes and in erythrocytes. These results show that ascorbate does not get lost but is effectively reutilized even in case of diminished recycling and it should be taken into account not only as a vitamin, but also as a source of energy. (Banhegyi, Braun, Csala, Puskas, Mandl, 1997) It would be hard to identify the metabolites of Vit c in the 1H nmr spectrum of urine after a prolonged high dosage of vitamin C as figure 6 shows a large peak of unmetabolised Vit c which is excreted in the urine. This peak, surrounded by intermediates of the pathway which breaks down ascorbate acid, dominates the 1H nmr spectrum and masks weaker signals. Therefore the metabolites which are produced by the breakdown of some of the Vit c are hard to identify. The common feature which persists throughout the degradative pathway is the CH2-CH molecular unit which is part of all the intermediates within the pathway, and this is the bit that gives the NMR fingerprint. This is shown in figure 6 with several smaller peaks around the Vit C peak. These are the intermediates of the pathway which contain the CH2-CH molecule which is present in the parent ascorbate acid and therefore have a similar structure and appear as peaks around Vit C. These will probably be intermediates in the pathway w hich breaks down ascorbate acid and contain the same CH2-CH molecular unit intact that was present in the parent ascorbate structure Comment on the human bodys requirement for vitamin C, its role in prevention/treatment of disease (briefly), the required daily intake/doseage, etc. How does this relate to the results dicussed above? Recommendations for vitamin C intake have been set by various national agencies: 75 milligrams per day: the United Kingdoms Food Standards Agency The key importance of Vitamin C is supporting the immune system and forming a structural component known as collagen. It is also required for synthesis of the neurotransmitter, required for brain function and mood change. Vitamin C aids in synthesis of a small molecule, carnitine. Carnitine is required for fat transportation to cellular organelles known as mitochondria, potentially, producing energy. Vitamin C has the ability to enhance bodys resistance to varied diseases. It aids in stimulating the action of antibodies and immune cells like phagocytes, resulting in a stronger immune system. Vitamin C metabolite L-threonic acid or its calcium salt, calcium threonate (the form of L-threonic acid found in Ester-C), increases vitamin C uptake of cells. Essentially, with calcium threonate, vitamin C has been shown to be absorbed more quickly, reach higher levels and is excreted more slowly. Now the studies confirm that the vitamin C uptake of the cells is greater with the metabolite L-threonic acid present. identify the advantages and disadvantages of using NMR over other common analytical methods used in Biomedical Sciences (or elsewhere). In NMR spectroscopy, only a very small excess of the spins are in the low energy state. The net result of this is that NMR is rather insensitive technique relative to many other analytical methods. Typically, even todays spectrometers require a minimum of several nanomoles of material for anaylsis in reasonable times. Poor sensitivity has been the bane of bioanalytical uses of NMR and increasing NMR sensitivity has been the focus of most of the technical developments that have occurred over the past four decades. However, in contrast to the low intrinsic sensitivity in the applications of NMR to biofluids, the non-selectivity of NMR makes it a very powerful tool for surveying the molecular content of a sample without prejudging which analytes to search for. This advantage can also be a nuisance. Scarce analytes often need to be measured and although above the limit of detection, these lower level species may be fully or partially obscured by analytes at much higher concentrations. (Gadian, 2004) A comparison of NMR spectroscopy with HPLC shows a variety of advantages of NMR over HPLC method. The primary advantage of NMR is its efficiency due to the lack of any preparation times. The analyte has to be weighed and dissolved in the solvent only and afterwards the analyte can be measured immediately. The experimental time depends on the concentration of the analyte. Using HPLC for the determination of an analyte much time has to be spent for the equilibration of the column. The column has to be washed every day after the measurements have been taken to prolong the lifetime of the column. When using the HPLC technique, often much time has to be spent for sample preparation e.g. derivatization of the analyte. A further disadvantage is the large amount of solvent necessary for the HPLC separation. NMR is also more efficient than the conventional HPLC techniques. (Wawer, Holzgrabe, Diehl, 2008)

Anarchy: Political Ideals To A Symbol Of Unconformity :: essays research papers fc

Anarchy: Political Ideals To A Symbol Of Uncoformity â€Å"Anarchism, then really stands for the liberation of human mind from the domination of religion, The liberation of the human body from the domination of property, Liberation from the shackles and restraints of government†#-Emma Golman. During the late 1800’s urbanization began to inflict the cities and the industrial revolution began resulting in governments gaining more and more power. â€Å"The state is authority; its force†#-Mikhail Bakunin. As the governments grew it was believed the state was more concerned with its growing power rather than the interests of the people. A group known as the anarchist believed that the government should be abolished and then the people would be free to live co-operatively with full social and political. Anarchy began as a political philosophy and soon turned in to an all out revolution resulting in assignations, bombings and kidnappings spanning over the better part of the past century. During the 1970’s and 1980à ¢â‚¬â„¢s, anarchy started to become more of a fashion trend if you will, rather than a political philosophy. â€Å"I Wanna Be Anarchy†-Sex Pistols. The Punk movement in music during the late 70’s was first to wide spread expose the public to anarchy and anarchist ideals. Followers of punk and punk music usually didn’t have the tendency to look of the proper meaning of anarchy, but since Johnny Rotten was saying it, it was cool. Today if you take a look at the public wither you are in a public school or a shopping mall, you can see teenagers with anarchy symbols on their shirts, pants, back packs and even drawn on their sneakers in an attempt to look what the public calls â€Å"hardcore†. â€Å"Anarchism is the sprit of the youth against out worn traditions†-Mikhail Bakunin, this would prove to be all too true in this new era of â€Å"anarchism†. This paper will further outline how anarchy started out as a political philosophy and turned in t o a symbol of unconformity.   Ã‚  Ã‚  Ã‚  Ã‚  Anarchism can be defined as a political philosophy and social movement designed to destroy the government in hopes of creating a society based on voluntary co-operation of free individuals. In 1840 Pierre-Joseph Proudhon, a peaceful anarchist, published his controversial pamphlet titled â€Å"What Is Property†. Proudhon clamed that violence and crime was not caused by individuals but instead by the government. He believed that police and laws forced humans to live in an unnatural state of oppression and equality, according to Proudhon the ownership of property was the main root of all equality.

Master of German Opera – Richard Wagner

Richard Wagner is the master of German opera. He molded opera according to his own creative definition with revolutionary zeal (Richard Wagner Biography, Arizona Opera). Adolph Hitler and his Nazi party enjoyed many of Wagner's works; this negative publicity has caused people hate Wagner and his music. Today Wagner is accepted, but his music is still outlawed in Israel. The youngest of nine children, Wagner was born in Leipzig, Germany to a blue-collar family, on May 22, 1813. His father, Friedrich Wagner, a police clerk, died when Richard was only six months old. His mother soon remarried to an actor and playwright named Ludwig Geyer and the family moved to Dresden. Some historians believe that Geyer was Richard's actual biological father; there is evidence that shows that Wagner may have believed this too. Egocentric from childhood, Wagner loved literature. He was especially fascinated by the works of Shakespeare. With his head always in the books, he tried writing tragedies but that never came to anything. He also began piano lessons, but unlike many other well-known composers, he never became skilled on this or any other instrument. Through his teens composing attracted him. In 1829, at age sixteen, he wrote his first compositions, which were not received well by the public. Then he took six months of formal training Theodor Weinlig, cantor of the Thomasschule, in 1831. Those studies resulted in the composition of a Wagner symphony, which was well received in Leipzig and Prague. He began work on an opera, Die Hochzeit, and tossed it aside unfinished. After that he completed Die Feen, but it was not performed until five years after his death. At 20, he began keeping journals in hopes of writing an autobiography in the future. He got a job as the chorus master for the Wurzburg Theatre when he was only 20 years old. He also started to write operas. In 1836, he got married to the actress, Minna Planner, this marriage was to last only until 1862. He was very unhappy with Minna, and regretted this marriage almost as soon as it came to be. In 1837, he and his wife escaped creditors to Paris. Wagner struggled to establish himself in Paris. Most of the time they were living on the verge of starvation; he was arrested and imprisoned on a few different occasions due to his debts. Producers rejected his preliminary ketches of the operas La Rienzi and Das Liebesverbot. Wagner stopped writing all together because of the embarrassment of being rejected. Then Wagner had a new idea and started work on The Flying Dutchman, even though he was impoverished and unknown he felt victorious at its completion in 1841. La Rienzi opened in Dresden in 1842 to enormous success. A success followed the next year for The Wagner became Kapellmeister of the Dresden opera and had financial security at last. However, he continued his poor money management, accumulating impossible debts. Within the five years that followed, he had completed Tannhauser, and Lohengrin. However, Lohengrin, which he considered his greatest effort to date, was rejected by Dresden opera and, in anger, Wagner helped to start a revolution. He wrote letters to Dresden rioters who were creating a growing rebellion in the state of Saxony. In 1849, when the revolution failed, Wagner was exiled, and forced to flee to Switzerland. In Zurich, 1851, he completed his furiously anti Semitic Jewishness in Music. During his thirteen-year exile, he concentrated on writing essays, which told his theories on musical drama. He also began work on his giant creation, a cycle of four musical dramas titled Der Ring es Nibelungen (The Ring of the Nibelungs). This cycle was made up of these four dramas Das Rheingold, Die Walkure, Siegfried and Die Gotterdammerung . Der Ring des Nibelungen took 22 years to complete, and stands as one of the most remarkable and extremely influential achievements in music. During the many years of Wagner's exile, Lohengrin was presented in Weimar and was received slowly just as Tannhauser had been. However, in the decade that followed, German audiences loved both operas. In fact, at the time his exile ended in 1860, Wagner was one of the few Germans who had never seen a performance of Lohengrin. During the long period of work on The Ring of the Nibelungs, Wagner's life went through some major changes. He wrote, maybe, his most popular opera, Tristan und Isolde in 1859, and another major work, Die Meistersinger Von Nurnberg in 1867. In 1864, the King of Bavaria, Ludwig II, brought Richard to Munich to provide financial support. There Wagner fell deeply in love with a daughter of Franz Liszt, Cosima von Bulow, who was married at the time to one of Wagner's associates. Eventually they got married. They began a project that would prove Wagner's biggest legacy to future generations (Richard Wagner, classicalmus. m), the building of an opera house that was devoted to only Wagner's works. Set in the Bavarian town Bayreuth, the Festspielhaus was the location for the first complete performance of the Ring cycle, in 1876. This was probably Wagner's biggest achievement, to have his greatest work performed in his own opera house. I don't personally listen to classic music; I don't enjoy a whole lot. I did, though, like Wagner's music. I didn't think that I would but I did. My favorite song was Lohengrin because it moves from very calm and quiet to very exciting and loud throughout the whole piece. Wagner has been classified politically as an anarchist and socialist, and simultaneously, a fascist, nationalist, and anti-Semite. His name has been connected to almost all the major trends in German history of the 19th and 20th centuries (Richard Wagner, Bena. com). Some people don't like him or his music just because some of the ideas that has been associated with his name. People need to look past the controversy and just listen to his music, and until people can do this, his wonderful music will never be fully enjoyed.

Personality Reflection Paper - 939 Words

Personality Reflection Paper Debbie Cooper PSY/250 July 30, 2012 AnneySnyder Personality Reflection Paper The following will reflect this researchers understanding and reflection on personality, how it defines one, what it means, and if one’s personality changes to fit certain situations. At the end this researcher may have a better insight into herself as well as the personalities of others. This insight can help her in her future endeavors. Personality I would define personality as what makes each individual different. Each human being’s personality is as unique as they are. While most people define personality to describe another’s more apparent characteristics, psychologists use personality to try to give reason to similar†¦show more content†¦I am dedicated because I am devoted to those I know and projects that I value, I complete what I start. I am patient because I am steadfast and understanding, it takes a lot for me to get flustered. Key Personality Concepts Some key personality concepts that define who I am is introvert, intuitive, intuitive feeling, and judging. Introverted intuition allows me to appreciate a better clarity of perception of inner unconscious techniques. It allows me to act spontaneously and insightful as original resolutions present themselves, on a case by case basis. Basically this means that I follow my intuition and not how I may sense something. Feeling allows me to express my emotions when others are involved. I also freely express my opinion when others are involved. At times I find myself torn between the moral conclusions about their actions and attitudes and my longing to express my bucket full of feelings and the consequences of the unbridled truth. My confidants are chosen with care, I understand that there are those out there who will stab you in the back without batting an eyelash. Judging was a concern for me, until a wise person informed us that it should not. It means that I listen without interruption to obtain the full amount of information before asking questions or giving my opinion. It does not mean that I judge – comment or criticize a person, their beliefs, or their opinion. Constant or Changing I believe that ourShow MoreRelatedReflection Paper On Personality1427 Words   |  6 PagesFor this paper, I wanted to get an analytical point of view on what my personality is. To do this, I took two personality tests from the Similar Minds website. The first one I took was the Big 45 Test. That personality test consisted of 225 questions that gave me the answer range from ‘very inaccurate’ to ‘very accurate’. My results were nearly similar in percentage; for Extroversion I got 59%, Accommodation is 67%, Orderliness is 59%, Emotional Stability is 51%, and Open-mindedness is 73%. 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The meanings alternate a littleRead MoreThe Reflection Of Charles Dickens Great Expectations1590 Words   |  7 Pages The Reflection of Charles Dickens in Great Expectations Introduction: The general definition of reflection is† to bring or cast as result â€Å". Dr. Harvey from Macquarie University define reflection as â€Å"an intended action, it depends on the past actions, present actions, and future actions.† In this paper we will see the reflection in â€Å"Art†. Art is the reflection of the identity and personality, for example in painting, drawing, writing, and many more. Art can symbolize the personality of the